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ENZYMES
Klenow DNA Polymerase (DR01101)
(DNA Polymerase I Large Fragment)
■ Description
Klenow DNA Polymerase is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3'→ 5' exonuclease activity, but has lost 5'→ 3' exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.
Klenow DNA Polymerase (3' → 5' exo -)
(DR01102)
■ Description
Klenow Fragment (3´→ 5´ exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5´→ 3´ exonuclease activity and has mutations which abolish the 3´→ 5´ exonuclease activity.
T4 DNA Ligase (DR01201)
■ Description
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
TEV Protease (DR01301)
■ Description
TEV Protease is a recombinant protease from Tobacco Etch Virus (TEV). It cleaves 7 amino acid sequences of (Glu-Asn-Leu-Tyr-Phe-Gln↓Gly) between Gln and Gly. TEV Protease of DoctorProtein has mutations to improve enzyme stability. Since TEV Protease contains 6X His tag sequence, it can be easily removed by a metal affinity chromatography.
Phosphatase (Antarctic) (DR01401)
■ Description
Phosphatase (Antarctic) is enzyme that catalyzes the removal of 5’ phosphate from DNA and RNA. Phosphatase treated DNA or RNA fragments cannot self-ligate. Because, they lack the 5´ phosphoryl termini required by ligase. Therefore, vector background can be decreased in cloning by phosphate treatment.
Phosphatase can be inactivated by simple heat treatment. After phosphatase treatment reaction mixture can be used directly further step without purification.
HRV 3C Protease (DR01501)
■ Description
HRV 3C Protease is a recombinant protease from human rhinovirus. It cleaves 7 amino acid sequences of (Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro) between Gln and Gly. HRV 3C Protease shows high efficiency at low temperature, it is suitable to remove fusion tags from recombinant proteins. Since HRV 3C Protease of DoctorProtein contains 6X His tag sequence, it can be easily removed by a metal affinity chromatography.